资源类型

期刊论文 38

年份

2023 4

2022 9

2021 6

2020 3

2019 4

2018 3

2017 2

2016 1

2015 2

2013 1

2012 1

2009 1

2008 1

展开 ︾

关键词

生物材料 2

CAR19 1

CART19 1

个人热管理 1

乳状液 1

仿生 1

仿生材料 1

仿生牙釉质 1

仿生纺丝 1

光遗传学 1

再生 1

再矿化 1

力学增强策略 1

功能仿生 1

功能表面 1

同轴管式电极 1

器官芯片 1

多级结构 1

嵌合抗原受体 1

展开 ︾

检索范围：

排序： 展示方式：

The development and application of genome editing technology in ruminants: a review

Mengke YUAN, Yuanpeng GAO, Jing HAN, Teng WU, Jingcheng ZHANG, Yongke WEI, Yong ZHANG

《农业科学与工程前沿（英文）》 2020年 第7卷 第2期   页码 171-180 doi: 10.15302/J-FASE-2019302

摘要：

Transgenic ruminants are a valuable resource for both animal breeding and biomedical research. The development of transgenic breeding is proceeding slowly, because it suffers from low efficiency of gene transfer and possible safety problems from uncontrolled random integration. However, new breeding methods combined with genome editing and somatic cell nuclear transfer or microinjection can offer an economic and efficient way to produce gene-edited ruminants, which can serve as bioreactors or have improved disease resistance, animal welfare and product quality. Recent advances in precise genome editing technologies, especially clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 nucleases, are enabling the systematic development of gene-edited ruminant production. This review covers the development of gene-edited ruminants, the particulars of site-specific engineered nucleases and the state of the art and new insights into practical applications and social acceptance of genome editing technology in ruminants. It is concluded that the production of gene-edited ruminants is feasible and through improvements in genome editing technology it is possible to help feed the world.

关键词： bioreactors     breeding     engineered endonucleases     genome editing     ruminants    

HTML PDF 收藏

Novel engineered proteins for mechanomaterials

Giuseppe Portale

《化学科学与工程前沿（英文）》 2020年 第14卷 第6期   页码 1122-1123 doi: 10.1007/s11705-020-1941-x

HTML PDF 收藏

Study of base friction simulation tests based on a complicated engineered bridge slope

Liu HE, Guang WU, Hua WANG

《结构与土木工程前沿（英文）》 2012年 第6卷 第4期   页码 393-397 doi: 10.1007/s11709-012-0174-6

摘要： In this paper, a physical base friction test model of a slope is established. The model is based on similarity principles and the geological conditions of a complicated bridge slope during construction, deformation and failure. The behavior of the slope in both its natural state and during excavation loading is qualitatively analyzed through base friction tests. The base friction test results are then subjected to comparison and analysis using finite element numerical simulation. The findings show that the whole engineered slope tends to stabilize in its natural state, whereas instabilities will arise at faulted rock masses located near bridge piers during excavation loading. Therefore, to ensure normal construction operation of bridge works, it is suggested that pre-reinforcement of faulted rock masses be performed.

关键词： base friction test     slope stability evaluation     bridge slope    

HTML PDF 收藏

Effect of natural pozzolan content on the properties of engineered cementitious composites as repair

Said CHOUCHA, Amar BENYAHIA, Mohamed GHRICI, Mohamed Said MANSOUR

《结构与土木工程前沿（英文）》 2018年 第12卷 第3期   页码 261-269 doi: 10.1007/s11709-017-0394-x

摘要：

In order to determine the effect of Natural Pozzolan (NP) content on the mechanical properties and durability characteristics on Engineered Cementitious Composites (ECC) as repair material. This study focused on the evaluation of the most factors influencing compatibility between the repair material and the base concrete including mechanicals properties such as, compressive and flexural strengths, elastic modulus, capillary absorption and drying shrinkage. The experimental results showed that natural pozzolan reduces the compressive strength and the flexural strength of ECC at all ages. The elastic modulus of ECC was remarkably lower than that of normal-strength concrete. This lower Young’s modulus is desirable for repair concrete, because it prevents the stresses induced by restrained shrinkage. In addition, the incorporation of high-volume natural pozzolan decreases significantly the coefficient of capillary absorption at long term and increases the drying shrinkage. Generally, based on the results obtained in the present experimental investigation, ECC can be used effectively as an overlay material over existing parent concrete.

关键词： natural pozzolan     engineered cementitious composites     mechanical strengths     elastic modulus     capillary absorption     drying shrinkage    

HTML PDF 收藏

Enhanced atrazine removal using membrane bioreactor bioaugmented with genetically engineered microorganism

LIU Chun, HUANG Xia

《环境科学与工程前沿（英文）》 2008年 第2卷 第4期   页码 452-460 doi: 10.1007/s11783-008-0050-8

摘要： Bioaugmentation with genetically engineered microorganisms (GEMs) in a membrane bioreactor (MBR) for enhanced removal of recalcitrant pollutants was explored. An atrazine-degrading genetically engineered microorganism (GEM) with green fluorescent protein was inoculated into an MBR and the effects of such a bioaugmentation strategy on atrazine removal were investigated. The results show that atrazine removal was improved greatly in the bioaugmented MBR compared with a control system. After a start-up period of 6 days, average 94.7% of atrazine was removed in bioaugmented MBR when atrazine concentration of influent was 14.5 mg/L. The volumetric removal rates increased linearly followed by atrazine loading increase and the maximum was 65.5 mg/(L·d). No negative effects were found on COD removal although carbon oxidation activity of bioaugmented sludge was lower than that of common sludge. After inoculation, adsorption to sludge flocs was favorable for GEM survival. The GEM population size initially decreased shortly and then was kept constant at about 10–10 CFU/mL. Predation of micro-organisms played an important role in the decay of the GEM population. GEM leakage from MBR was less than 10 CFU/mL initially and was then undetectable. In contrast, in a conventionally activated sludge bioreactor (CAS), sludge bulking occurred possibly due to atrazine exposure, resulting in bioaugmentation failure and serious GEM leakage. So MBR was superior to CAS in atrazine bioaugmentation treatment using GEM.
HTML PDF 收藏

Preparation and characterization of hydrothermally engineered TiO

Kanchapogu Suresh, G. Pugazhenthi, R. Uppaluri

《化学科学与工程前沿（英文）》 2017年 第11卷 第2期   页码 266-279 doi: 10.1007/s11705-017-1608-4

摘要： This work targets the preparation and characterization of an inexpensive TiO -fly ash composite membrane for oily wastewater treatment. The composite membrane was fabricated by depositing a hydrophilic TiO layer on a fly ash membrane via the hydrothermal method, and its structural, morphological and mechanical properties were evaluated. The separation potential of the composite membrane was evaluated for 100–200 mg·L synthetic oily wastewater solutions. The results show that the composite membrane has excellent separation performance and can provide permeate stream with oil concentration of only 0.26–5.83 mg·L . Compared with the fly ash membrane in the average permeate flux and performance index (49.97 × 10 m ·m ·s and 0.4620%, respectively), the composite membrane exhibits better performance (51.63 × 10 m ·m ·s and 0.4974%). For the composite ash membrane, the response surface methodology based analysis inferred that the optimum process parameters to achieve maximum membrane flux and rejection are 207 kPa, 200 mg·L and 0.1769 m·s for applied pressure, feed concentration and cross flow velocity, respectively. Under these conditions, predicted responses are 41.33 × 10 m ·m ·s permeate flux and 98.7% rejection, which are in good agreement with the values obtained from experimental investigations (42.84 × 10 m ·m ·s and 98.82%). Therefore, we have demonstrated that the TiO -fly ash composite membrane as value added product is an efficient way to recycle fly ash and thus mitigate environmental hazards associated with the disposal of oily wastewaters.

关键词： TiO2-fly ash membrane     oily wastewater     fouling     microfiltration    

HTML PDF 收藏

Activated carbon induced oxygen vacancies-engineered nickel ferrite with enhanced conductivity for supercapacitor

《化学科学与工程前沿（英文）》 2023年 第17卷 第12期   页码 2088-2100 doi: 10.1007/s11705-023-2352-6

摘要： Activated carbon induced oxygen vacancies-engineered nickel ferrite with enhanced conductivity for supercapacitor application

关键词： nickel ferrite conductivity     carbon oxygen vacancies    

HTML PDF 收藏

Research progress of defect-engineered UiO-66(Zr) MOFs for photocatalytic hydrogen production

《能源前沿（英文）》 2021年 第15卷 第3期   页码 656-666 doi: 10.1007/s11708-021-0765-9

摘要： In recent years, defect-engineered Zr-based UiO-66 metal-organic frameworks (UiO-66(Zr) metal-organic frameworks (MOFs)) have shown huge advantages in catalytic, functional materials, adsorption, and other fields due to their large surface areas, well-ordered porous structures, and flexible tailorability. It is extremely challenging to introduce defect sites in the synthesis of MOFs to regulate the physicochemical properties of materials such as (energy band structure, pore structure, etc.) to obtain an excellent performance. This paper reviews the recent research results of synthesis methods, characterization technologies, and application fields of defect-engineered UiO-66(Zr) MOFs materials in order to provide new insights to synthesize high-performance UiO-66(Zr) MOFs materials and promote the development of UiO-66(Zr) in various fields.

关键词： defect engineering     metal-organic frameworks     UiO-66     photocatalysis    

HTML PDF 收藏

Synthetically engineered microbes reveal interesting principles of cooperation

Michael D. Dressler,Corey J. Clark,Chelsea A. Thachettu,Yasmine Zakaria,Omar Tonsi Eldakar,Robert P. Smith

《化学科学与工程前沿（英文）》 2017年 第11卷 第1期   页码 3-14 doi: 10.1007/s11705-016-1605-z

摘要： Cooperation is ubiquitous in biological systems. However, if natural selection favors traits that confer an advantage to one individual over another, then helping others would be paradoxical. Nevertheless, cooperation persists and is critical in maintaining homeostasis in systems ranging from populations of bacteria to groupings of mammals. Developing an understanding of the dynamics and mechanisms by which cooperation operates is critical in understanding ecological and evolutionary relationships. Over the past decade, synthetic biology has emerged as a powerful tool to study social dynamics. By engineering rationally controlled and modulatable behavior into microbes, we have increased our overall understanding of how cooperation enhances, or conversely constrains, populations. Furthermore, it has increased our understanding of how cooperation is maintained within populations, which may provide a useful framework to influence populations by altering cooperation. As many bacterial pathogens require cooperation to infect the host and survive, the principles developed using synthetic biology offer promise of developing novel tools and strategies to treat infections, which may reduce the use of antimicrobial agents. Overall, the use of engineered cooperative microbes has allowed the field to verify existing, and develop novel, theories that may govern cooperative behaviors at all levels of biology.

关键词： synthetic biology     engineered bacteria     cooperation     cheater     quorum sensing    

HTML PDF 收藏

diversity of NO reductases, new tools for monitoring, and insights into NO production in natural and engineered

《环境科学与工程前沿（英文）》 2022年 第16卷 第10期 doi: 10.1007/s11783-022-1562-3

摘要：

● 548 representative nor genes were collected to create complete phylogenetic trees.

关键词： N2O     Greenhouse gas     NO reductase     NO dismutase     Primer     Crystal structure    

HTML PDF 收藏

转基因作物

Lance A. Davis

《工程（英文）》 2016年 第2卷 第3期   页码 268-269 doi: 10.1016/J.ENG.2016.03.007

HTML PDF 收藏

Photocatalytic degradation of the acetaminophen by nanocrystal-engineered TiO

Reza Katal, Mohammad Tanhaei, Jiangyong Hu

《环境科学与工程前沿（英文）》 2021年 第15卷 第2期 doi: 10.1007/s11783-020-1319-9

摘要： Abstract • Photocatalytic activity was improved in TiO2 thin film by rapid thermal annealing. • Photoreactor was designed for TiO2 thin film. • Considerable reusability and durability of prepared photocatalysts were studied. Un-biodegradable pharmaceuticals are one of the major growing threats in the wastewaters. In the current study, TiO2 thin film photocatalysts were designed by nanocrystal engineering and fabricated for degradation of the acetaminophen (ACE) in a photocatalytic reaction under UV light irradiation in batch and continuous systems. The photocatalyst was prepared by sputtering and then engineered by thermal treatment (annealing at 300℃ (T300) and 650℃ (T650)). The annealing effects on the crystallinity and photocatalytic activity of the TiO2 film were completely studied; it was found that annealing at higher temperatures increases the surface roughness and grain size which are favorable for photocatalytic activity due to the reduction in the recombination rate of photo-generated electron-hole pairs. For the continuous system, a flat plate reactor (FPR) was designed and manufactured. The photocatalytic performance was decreased with the increase of flow rate because the higher flow rate caused to form the thicker film of the liquid in the reactor and reduced the UV light received by photocatalyst. The reusability and durability of the samples after 6 h of photocatalytic reaction showed promising performance for the T650 sample (annealed samples in higher temperatures).

关键词： Acetaminophen     TiO2     Thin film     Batch     Continuous    

HTML PDF 收藏

Efficient acetoin production from pyruvate by engineered whole-cell biocatalysis

《化学科学与工程前沿（英文）》 2023年 第17卷 第4期   页码 425-436 doi: 10.1007/s11705-022-2229-0

摘要： Acetoin is an important platform chemical, which has a wide range of applications in many industries. Halomonas bluephagenesis, a chassis for next generation of industrial biotechnology, has advantages of fast growth and high tolerance to organic acid salts and alkaline environment. Here, α-acetolactate synthase and α-acetolactate decarboxylase from Bacillus subtilis 168 were co-expressed in H. bluephagenesis to produce acetoin from pyruvate. After reaction condition optimization and further increase of α-acetolactate decarboxylase expression, acetoin production and yield were significantly enhanced to 223.4 mmol·L–1 and 0.491 mol·mol–1 from 125.4 mmol·L–1 and 0.333 mol·mol–1, respectively. Finally, the highest titer of 974.3 mmol·L–1 (85.84 g·L–1) of acetoin was accumulated from 2143.4 mmol·L–1 (188.6 g·L–1) of pyruvic acid within 8 h in fed-batch bioconversion under optimal reaction conditions. Moreover, the reusability of the cell catalysis was also tested, and the result illustrated that the whole-cell catalysis obtained 433.3, 440.2, 379.0, 442.8 and 339.4 mmol·L–1 (38.2, 38.8, 33.4, 39.0 and 29.9 g·L–1) acetoin in five repeated cycles under the same conditions. This work therefore provided an efficient H. bluephagenesis whole-cell catalysis with a broad development prospect in biosynthesis of acetoin.

关键词： acetoin     pyruvate     α-acetolactate synthetase     α-acetolactate decarboxylase     Halomonas bluephagenesis     whole-cell biocatalysis    

HTML PDF 收藏

Controlling various contaminants in wastewater effluent through membranes and engineered wetland

Sarper SARP, Sungyun LEE, Noeon PARK, Nguyen Thi HANH, Jaeweon CHO

《环境科学与工程前沿（英文）》 2009年 第3卷 第1期   页码 98-105 doi: 10.1007/s11783-009-0006-7

摘要： For effective wastewater reclamation and water recovery, the treatment of natural and effluent organic matters (NOM and EfOM), toxic anions, and micropollutants was considered in this work. Two different NOM (humic acid of the Suwannee River, and NOM of US and Youngsan River, Korea), and one EfOM from the Damyang wastewater treatment plant, Korea, were selected for investigating the removal efficiencies of tight nanofiltration (NF) and ultrafiltration (UF) membranes with different properties. Nitrate, bromate, and perchlorate were selected as target toxic anions due to their well known high toxicities. Tri-(2-chloroethyl)-phosphate (TCEP), oxybenzone, and caffeine, due to their different and p values, were selected as target micropollutants. As expected, the NF membranes provided high removal efficiencies in terms of all the tested contaminants, and the UF membrane provided fairly high removal efficiencies for anions (except for nitrate) and the relatively hydrophobic micropollutant, oxybenzon

关键词： wastewater reclamation     natural organic matter (NOM)     effluent organic matter (EfOM)     membranes     wetlands    

HTML PDF 收藏

Efficient production of D-1,2,4-butanetriol from D-xylose by engineered

Shewei Hu, Qian Gao, Xin Wang, Jianming Yang, Nana Xu, Kequan Chen, Sheng Xu, Pingkai Ouyang

《化学科学与工程前沿（英文）》 2018年 第12卷 第4期   页码 772-779 doi: 10.1007/s11705-018-1731-x

摘要： We have developed a whole-cell bioconversion system for the production of D-1,2,4-butanetriol (BT) from renewable biomass. A plasmid pETduet- T7- T7- was constructed and transformed to BL21(DE3) to obtain the whole cells of BL21- capable of bioconversion D-xylose to BT. Then, the factors including carbon sources, nitrogen sources, metal ions, and culture conditions (pH, temperature, IPTG) were identified, and their effects on the whole-cell activity for BT production were investigated. To obtain the highest whole-cell activity, the optimal cultivation parameters are: 15 g·L yeast extract, 5 g·L sucrose, 3g·L KH PO , 5 g·L NaCl, 3g·L NH Cl, 0.25 g·L MgSO ?7H O and 1 mL·L the mixture of trace elements. With the optimized whole cells of BL21- , 60 g·L of xylose was converted to 28g·L BT with a molar yield of 66 %, which is higher than those reported in the biotechnological system.

关键词： D-1     2     4-butanetriol     whole-cell bioconversion     carbon source     nitrogen sources     metal ions     culture conditions    

HTML PDF 收藏

标题 作者 时间 类型 操作

The development and application of genome editing technology in ruminants: a review

Mengke YUAN, Yuanpeng GAO, Jing HAN, Teng WU, Jingcheng ZHANG, Yongke WEI, Yong ZHANG

期刊论文

Novel engineered proteins for mechanomaterials

Giuseppe Portale

期刊论文

Study of base friction simulation tests based on a complicated engineered bridge slope

Liu HE, Guang WU, Hua WANG

期刊论文

Effect of natural pozzolan content on the properties of engineered cementitious composites as repair

Said CHOUCHA, Amar BENYAHIA, Mohamed GHRICI, Mohamed Said MANSOUR

期刊论文

Enhanced atrazine removal using membrane bioreactor bioaugmented with genetically engineered microorganism

LIU Chun, HUANG Xia

期刊论文

Preparation and characterization of hydrothermally engineered TiO

Kanchapogu Suresh, G. Pugazhenthi, R. Uppaluri

期刊论文

Activated carbon induced oxygen vacancies-engineered nickel ferrite with enhanced conductivity for supercapacitor

期刊论文

Research progress of defect-engineered UiO-66(Zr) MOFs for photocatalytic hydrogen production

期刊论文

Synthetically engineered microbes reveal interesting principles of cooperation

Michael D. Dressler,Corey J. Clark,Chelsea A. Thachettu,Yasmine Zakaria,Omar Tonsi Eldakar,Robert P. Smith

期刊论文

diversity of NO reductases, new tools for monitoring, and insights into NO production in natural and engineered

期刊论文

转基因作物

Lance A. Davis

期刊论文

Photocatalytic degradation of the acetaminophen by nanocrystal-engineered TiO

Reza Katal, Mohammad Tanhaei, Jiangyong Hu

期刊论文

Efficient acetoin production from pyruvate by engineered whole-cell biocatalysis

期刊论文

Controlling various contaminants in wastewater effluent through membranes and engineered wetland

Sarper SARP, Sungyun LEE, Noeon PARK, Nguyen Thi HANH, Jaeweon CHO

期刊论文

Efficient production of D-1,2,4-butanetriol from D-xylose by engineered

Shewei Hu, Qian Gao, Xin Wang, Jianming Yang, Nana Xu, Kequan Chen, Sheng Xu, Pingkai Ouyang

期刊论文